Product Guideline of Himedia Salmonella Shigella Agar 500gm

Himedia Salmonella Shigella Agar 500gm is prescribed as a differential and particular medium for the confinement of Salmonella and Shigella species from neurotic examples and suspected foodstuffs and for microbial constrain tests. It is a tolerably particular medium in which gram-positive microscopic organisms are hindered by bile salts, brilliant green, and sodium citrate.

Specification of Himedia Salmonella Shigella Agar 500gm

Product Type : SS Agar (Salmonella Shigella Agar)
Brand : HIMEDIA
Origin : USA ( Manufacturing Country in India )
Pack Size : 500gm
REF : 108-500g

Composition of Himedia Salmonella Shigella Agar 500gm

Peptone 5.000 Gms/Liter
HM peptone B # 5.000 Gms/Liter 
Lactose 10.000 Gms/Liter 
Bile salts mixture 8.500 Gms/Liter 
Sodium citrate 10.000 Gms/Liter 
Sodium thiosulphate 8.500 Gms/Liter 
Ferric citrate 1.000 Gms/Liter 
Brilliant green 0.00033 Gms/Liter 
Neutral red 0.025 Gms/Liter 
Agar 15.000 Gms/Liter 
Final pH ( at 25°C) 7.0±0.2 Gms/Liter 
Formula adjusted, standardized to suit performance parameters

Directions of Salmonella Shigella Agar:

Suspend 63.02 Grams In 1000 Ml Filtered /Refined Water. Bubble With Visit Tumult To Break Up The Medium Completely.
Do Not Autoclave Or Overheat. Cool To Around 50°c. Blend And
Pour Into Sterile Petri Plates.

Principle And Interpretation 

SS Media Agar medium is prescribed as a differential and particular medium for the confinement of Salmonella and Shigella species from neurotic examples (1) and suspected foodstuffs (2,3,4,5) and for microbial constrain test (6). SS Agar is a moderately selective medium in which gram-positive microbes are repressed by bile salts, brilliant green and sodium citrate.

Peptone, HM peptone B gives nitrogen and carbon sources, long-chain amino acids, vitamins and fundamental growth nutrients.

Lactose is a fermentable carbohydrate. Brilliant green, bile salts and thiosulphate specifically repress gram-positive and coliform living beings. Sodium thiosulphate is diminished by certain species of enteric life forms to sulphite and H2S gas and
this reductive protein handle is credited to thiosulphate reductase.

Conclusion  

The tall selectivity of Salmonella Shigella Agar permits the utilize of expansive inoculum a specifically from defecation, rectal swabs or other materials suspected of containing pathogenic enteric bacilli. On maturation of lactose by a few lactose-fermenting normal intestinal vegetation, corrosive is delivered which is shown by the alter of color from yellow to ruddy by the pH indicator-neutral red.

Thus these living beings develop as red-pigmented colonies. Lactose non-fermenting life forms develop as translucent colorless colonies with or without dark centers.